Bhakti Patel

6 Questions 19 Answers 0 Followers

Questions related from Bhakti Patel

Can anyone help me decide best combination of multicolor panels for my hematopoietic progenitor analysis from mouse bone marrow by FACS?

So basically, I am depleting lineage positive population from isolated bone marrow cells using MACS and then staining the lineage negative population with cocktail of fluorochrome tagged...

16 November 2018 5,543 10 View

What are best possible ways to attain full length cloning of an eukaryotic gene?

Initially I had succesfully cloned 526bp, 425bp and a 256bp target cDNA into a pGEMT easy vector systems (Promega). Sequencing results were found to be positive on BLAST search. Then, in oder to...

14 October 2015 6,671 5 View

Can anyone suggest B cell markers which could be helpful for B cell separation from whole blood of carp (Teleost fish)?

I need to separate B lymphocytes from blood and maintain a cell line of it. Regarding that how should I proceed with B cell separtion from whole blood of a teleost fish of Cyprinidae family. For...

23 April 2015 5,140 4 View

What is the reason for different Ct values in case of B-actin for 14 different cDNAs in qRT-PCR?

I have set of 7 tissues each for treatment and control groups. RNA integrity and concentration for all 14 tissues is good. Further, I treated with DNase 1 prior to cDNA synthesis. We use RevertAid...

10 September 2014 4,803 9 View

How can I identify know intron and exon regions in a given nucleotide sequence? How does a SignalP work?

Suppose I have sequenced one of my clones, how can I know which regions constitute the exon, intron, splice sites, splice variants, signal peptides and disufide bridges? What is the easiest method...

16 June 2014 8,001 5 View

How do I design primers for RACE amplification? What are the parameters for the same? How does it help in full length amplification of the gene?

I need to know how to design GSPs for RACE. Iam going to use Clontech Smarter RACE kit for the first time. My RNA quality is good. Even the GSPs I designed for semi-quantitative works well....

25 January 2014 2,377 8 View