Betül Uslu

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Questions related from Betül Uslu

Anyone could help me regarding rolling and wrinkling problem during frozen sectioning via sledge microtome?

Following fixation for 18-24h I store the whole brains in PBS containing Na-azide at 4C. On the sectioning day, I placed the brain on the freezing stage using couple drops of OCT applied to the...

11 September 2023 3,410 3 View

How can I ensure the tissue maintains its normal mobility in long-term organ bath experiments?

I am currently conducting experiments using organ baths with rat duodenum and ileum tissues. These experiments involve the administration of various drugs, including Fluoroacetate, Bethanechol,...

28 August 2023 410 3 View

What conditions should the remaining virus be stored after the AAV2-Retro has been used?

I'm working on microinjection. I wonder that how would- storage AAV2-Retro if it was not totally over in the pipette? Normally, we storage it in aliquots at -80. The virus I'm using now is left in...

06 August 2023 2,618 3 View