Bernadeta Dadonaite

3 Questions 1 Answers 0 Followers

Questions related from Bernadeta Dadonaite

Does anyone know if biotinylation of RNA decreases the efficiency of RNA extraction from cells with Trizol?

I've noticed that cells that have biotinylated RNA in them yield around 4 times less RNA if I'm using TRI Reagent (sigma) for RNA extraction. Anyone had a similar observation?

09 August 2016 374 1 View

What is the cause of smearing in 32P labeled RNA ladder?

Hi, I'm trying to label an RNA ladder (RiboRuler high range from thermofisher) but I get this smear (see image), which I cannot get rid of, perhaps someone has any suggestions as to what...

14 May 2016 3,960 3 View

Is it possible to recover silver stained RNA?

Does anyone know if it is possible to recover RNA from acrylamide gel that has been silver stained? The recovered RNA would then be used either in PCR reaction or loading again onto another gel.

21 March 2016 1,640 3 View