Benjamin Jurek

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Questions related from Benjamin Jurek

How to create a stably transfected neuronal cell line without lentivirus?

Hi, we want to transfect a neuronal cell line with our gene of interest, so that it is permanently expressed. The problem we face is that either we can transfect many cells with the help of AAVs,...

01 January 2019 2,470 19 View

What is the normal ratio of transposase helper plasmid to donor plasmid?

We want to transfect rat neuronal cells with VectorBuilder PiggyBac plasmids and the according helper plasmid. The company does not give a protocol. We've seen 1:1 1:4 or 1:5 ratios in literature....

07 July 2018 8,167 2 View