Benjamin Strobel

4 Questions 11 Answers 0 Followers

Questions related from Benjamin Strobel

Which mouse organ-isolated cells are best to FACS-sort with regard to isolation/dissociation, autofluorescence, etc.?

When studying reporter gene expression in mice by isolating cells that express a reporter gene (let’s say GFP, dsRed or other fluorescent reporters) and subsequent FACS analysis/sorting, would...

20 January 2016 3,414 3 View

What are the intracellular levels of guanine?

Hi everybody, I am wondering whether anyone is aware about the intracellular levels (molarity) of guanine in normal, healthy cells. I would be particularly interested in the levels measured in...

23 April 2015 2,951 1 View

Is there available pharmacokinetics / toxicity data for guanine application in mice?

I was looking for published PK and toxicity data for application of guanine in mice, ideally via different routes (oral, i.p., i.v.). Is anybody aware of available data?Thanks in advance.

13 August 2014 858 0 View

Why is there a higher amount of an unspecific PCR band with decreasing template DNA amounts?

When amplifying DNA from a plasmid by PCR (in order to add some restriction sites for cloning), we see a shift from the expected target band when using 1 µg template DNA, to increasingly higher...

17 June 2014 8,501 9 View