Beatriz Martín

2 Questions 4 Answers 0 Followers

Questions related from Beatriz Martín

How to remove the excess of biotin from the biotilanyted antibody after the bioconjugation step without losing antibody?

I am using the EZ-Link™ Sulfo-NHS-Biotinylation Kit from Thermo Fisher in order to get biotinylated antibodies. The kit seems to work well, but I have problems when removing the excess of unbound...

21 February 2019 1,773 9 View

Should I really see a pellet when I use the Exoquick-TC kit (SBI) to purify exosomes from cell culture?

I am trying to get exosomes from MDCK and Balbc 3T3 lines using the Exoquick-TC kit from SBI. At first I tried with T 75 flasks (that means I collect 6 ml of culture media -serum free-/flask), but...

18 April 2018 7,893 5 View