Beatriz Honorato

3 Questions 1 Answers 0 Followers

Questions related from Beatriz Honorato

Do human signal peptides work in mouse cells?

I am new at designing chimeric proteins and vector plasmids, and I need to make a murine cell line (MC38), excrete a protein that, usually, is in part excreted and in part retained in the nucleus....

03 June 2020 4,794 0 View

Is it too troublesome to use WPRE sequences in an insert in a AAV vector?

We have several chimeric proteins we want to increase the expression in our systems. We want ti use AAV vectors in mice, but we do not know if it would enlarge our inserts too much (they are...

07 September 2018 7,334 2 View

If HEK293 does not express TLR4 how is it that we detect TLR4 expression by RT-PCR in HEK293 virgin cells?

We need a model to transfect TLR4 and all sources say that HEK293 is the one, but when we are looking to confirm it, we detect TLR4 mRNA and we do not find the bibliographic source that says...

18 December 2017 6,253 3 View