7 Questions 16 Answers 0 Followers
Questions related from Bastian Fischer
Dear all, I have a question concerning AAV-transduction. I would like to use the AAV System to introduce a KO in NHDF-cells. For this, I transfected HEK293T-cells with 3 different Plasmids (see...
01 January 2018 4,078 3 View
Dear all, I try to detect possible mutations within my DNA. For this, I use the Surveyor Nuclease S Assay (Cel-I-Assay). After heteroduplex Formation andTreatment with Surveyor Nuclease, I don´t...
06 June 2016 8,067 3 View
Hey,I try to transfect primary human dermal fibroblasts. For this, I used several transfection reagents. One of it is DNA-IN (amsbio). A few cells are definitely transfected (strong GFP...
04 April 2016 4,271 5 View
Dear all, I would like to transfect primary cells (fibroblasts). The plasmid, which I would like to introduce, has a size of >8000 bp. Until now, I tried different transfection reagents for...
03 March 2016 2,899 12 View
Hey, I try to seperate different pyrophosphates via UPLC-MS/MS. For sure, their structure is very similar. For all of them, I measured the same product-ion...Is this a problem? I guess, it isn´t,...
11 November 2015 9,886 4 View
Dear all, I have got problems with my Western Blots. I try to detect the LDL - receptor. For this, I use an AB from Biovision (http://www.biovision.com/ldlr-antibody-1296.html), where I expect a...
05 May 2015 7,153 15 View
Dear all, I try to prove the presence of the LDL - receptor. I performed my Western Blot several times, with different antibodies and I always received the same bands. One of these bands (between...
05 May 2015 382 4 View
