Bas Luesken

3 Questions 5 Answers 0 Followers

Questions related from Bas Luesken

Does anyone have experience with detection of fungus Trichoderma harzianum through qPCR?

I need to detect the fungus Trichoderma harzianum with a qPCR. I know the strain (Trichoderma harzianum Rifai T22), but when I search the NCBI website for primer development I get stuck. When I...

25 October 2013 8,909 5 View

Why doesn't a qPCR reaction need to have an Elongation step?

Why doesn't a qPCR use an elongation step while a normal PCR reaction does next to its hybridization step? Is it because the fragments are smaller in a qPCR? Thank you for your time, Bas

16 July 2013 2,074 3 View

Straight line curve in q-PCR.

Attached is my file from a Biorad CFX96 Real Time system. The red lines are standard curves, the blue curves are monsters with different concentrations and the green curve is a negative...

27 May 2013 3,395 18 View