Bahja Al-Riyami

3 Questions 13 Answers 0 Followers

Questions related from Bahja Al-Riyami

What can I do to improve my protein purification?

I am trying to purify a protein for crystallization purpose, so I need an ultra-pure product. I expressed the protein as MBP-GFP fusion with His tag (at the C-terminal). The purification scheme...

10 October 2015 3,251 13 View

To which buffer should I dialyze my protein to avoid precipitation?

I purified my His tagged recombinant protein using affinity chromatography, followed by cation exchange chromatography and finally gel filtration. After gel filtration I started concentrating my...

11 November 2014 6,374 12 View

How can one store purified TEV protease?

I could not store aliquots at -20C or -80C as it precipitates when thawed. Do you usually add glycerol to your protein aliquots before freezing? Is it possible to store it without decreasing its...

10 October 2014 2,622 8 View