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Questions related from Bager Kalu
Hello I am testing different groups of T-cells (1,2,3 and 4) for the level of CD69 upregulation after stimulation with a molecule at different concentrations 1,2,3 and 4, see the attached plot....
29 November 2020 1,812 4 View
Hello there, I'm doing a western blot of my cells looking for binding of cd86 antibody. I am looking if my cells can bind the cd86 protein and then with the cd86 antibody. So is it possible to...
05 May 2018 4,962 1 View
Hi, We do transduction on t cells to express a specific target domain. The plasmid expressing the domain has a selecting marker. After selecting the tranduced cells using a specific isolation kit,...
05 May 2017 9,046 4 View
04 May 2017 4,898 4 View
Hello, I am working with chimeric antigen receptor and I know the difference in binding without MHC restriction for CARs and know the process of T cell activation and signaling process. But the...
11 November 2016 1,821 1 View
Hi I did isolation of PBMCs from whole blood through ficoll and activated them using anti-cd3/cd28 antibodies,after several days I did staining using flow cytomtery with cd4 and cd8...
11 November 2016 9,031 5 View
Hello,When doing retroviral transfection of 293T cells, according to the protocol there are two media; one has pH 7.1 and this one will be mixed with the plasmid DNA and the other medium has pH...
10 October 2016 6,439 2 View
