Bager Kalu

8 Questions 7 Answers 0 Followers

Questions related from Bager Kalu

What is the best statistical test for this experiment? two- or one-way anova?

Hello I am testing different groups of T-cells (1,2,3 and 4) for the level of CD69 upregulation after stimulation with a molecule at different concentrations 1,2,3 and 4, see the attached plot....

29 November 2020 1,825 4 View

Is it possible to blot the cell lysate with protein first then the antibody in western blot?

Hello there, I'm doing a western blot of my cells looking for binding of cd86 antibody. I am looking if my cells can bind the cd86 protein and then with the cd86 antibody. So is it possible to...

05 May 2018 4,971 1 View

Why do we use irradiated PBMCs to feed transduced t cells?

Hi, We do transduction on t cells to express a specific target domain. The plasmid expressing the domain has a selecting marker. After selecting the tranduced cells using a specific isolation kit,...

05 May 2017 9,058 4 View

Why do we use irradiated PBMCs to feed transduced t cells?

Hi, We do transduction on t cells to express a specific target domain. The plasmid expressing the domain has a selecting marker. After selecting the tranduced cells using a specific isolation kit,...

04 May 2017 4,911 4 View

What are the possible explanation for getting double positive and double negative populations in flow cytometry ?

Hi I did isolation of PBMCs from whole blood through ficoll and activated them using anti-cd3/cd28 antibodies,after several days I did staining using flow cytomtery with cd4 and cd8...

30 November 2016 305 6 View

What is the difference between TCRs and CARs in terms of signalling?

Hello, I am working with chimeric antigen receptor and I know the difference in binding without MHC restriction for CARs and know the process of T cell activation and signaling process. But the...

11 November 2016 1,836 1 View

What are the possible explanation for getting double positive and double negative populations in flow cytometry ?

Hi I did isolation of PBMCs from whole blood through ficoll and activated them using anti-cd3/cd28 antibodies,after several days I did staining using flow cytomtery with cd4 and cd8...

11 November 2016 9,048 5 View

Why do we use two different PHs and media during transfection?

Hello,When doing retroviral transfection of 293T cells, according to the protocol there are two media; one has pH 7.1 and this one will be mixed with the plasmid DNA and the other medium has pH...

10 October 2016 6,459 2 View