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Questions related from Aziz Ul Ikram
I tired different concentrations of Ligand (DNA) and protein, (400 µM, 30 µM), (200 µM, 10 µM),(200 µM, 5 µM), (100 µM, 5 µM). The data is improved but still the saturation point does not reach...
22 December 2020 9,729 8 View
Seeds obtained from heterozygous T0 mutants obtained through CRISPR CAS9 were sown and the plants raised were properly genotyped. Following normal segregation pattern, T1 generation had WT,...
20 September 2019 2,123 4 View
Recently I tried to knock out a gene in rice using CRISPR CAS9. I got heterozygous plants with different mutations combination like (-3,-1) and (-6,-2) in T0 (I confirmed the genotype of T0 by TA...
17 April 2018 5,599 5 View
I am using CRISPR/CAS9 system to knockout genes in rice. I have successfully used to knockout other genes. There is problem with only one gene. After checking and double checking, I see nothing...
25 February 2018 6,341 1 View
