Aziz Ul Ikram

4 Questions 4 Answers 0 Followers

Questions related from Aziz Ul Ikram

The saturation point does not reach in my ITC. Does this indicate that the interaction is weak or there is no interaction?

I tired different concentrations of Ligand (DNA) and protein, (400 µM, 30 µM), (200 µM, 10 µM),(200 µM, 5 µM), (100 µM, 5 µM). The data is improved but still the saturation point does not reach...

22 December 2020 9,715 8 View

How homozygous rice plants can produce heterozygous seeds?

Seeds obtained from heterozygous T0 mutants obtained through CRISPR CAS9 were sown and the plants raised were properly genotyped. Following normal segregation pattern, T1 generation had WT,...

20 September 2019 2,115 4 View

What are the possible reasons for non-transmission of a mutation to the next generation??

Recently I tried to knock out a gene in rice using CRISPR CAS9. I got heterozygous plants with different mutations combination like (-3,-1) and (-6,-2) in T0 (I confirmed the genotype of T0 by TA...

17 April 2018 5,591 5 View

Why my CRISPR/CAS9 system is unable to mutate the target gene?

I am using CRISPR/CAS9 system to knockout genes in rice. I have successfully used to knockout other genes. There is problem with only one gene. After checking and double checking, I see nothing...

25 February 2018 6,336 1 View