Azaz Ahmad

6 Questions 23 Answers 0 Followers

Questions related from Azaz Ahmad

Can there be genomic DNA contamination in an RNA sample?

I need your input to know whether my extracted RNA samples have genomic DNA contamination present or not and how the integrity of the samples is? I have nice 28s and 18s bands and the RNA...

05 May 2014 4,791 19 View

Problem with Western blot protein transfer

I have a problem with not properly transferring proteins on Nitrocellulose membrane. The transfer protein look fuzzy and all over the membrane as you can see in the picture. The protein marker was...

11 November 2013 3,042 17 View

I have been told to generate a standard curve on real-time qPCR. Does anyone have protocol or can roughly explain how it works?

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06 June 2013 7,970 19 View

Amount of sample load on gel during Western Blot?

I have some confusion about the amount of sample normally required to load on gel during Western blot. I will be loading 50ug of sample in total volume of 20ul (including loading buffer) ....

05 May 2013 6,306 18 View

Reverse Transcription

I have a problem with Reverse Transcription. Actually RT-PCR is not giving me bands after using both Clathrin and my target sequence primer sox1. I had included gDNA and got a band for my target...

03 March 2013 6,270 18 View

What lysis buffer is best for extracting proteins from fresh mouse brain tissue for western blot?

I will be using RIPA lysis buffer for other cells as mouse brain tissue has a lot of fats so my worry is will RIPA buffer work best on brain tissue? Another thing is if someone has any idea which...

02 February 2013 5,643 5 View