6 Questions 23 Answers 0 Followers
Questions related from Azaz Ahmad
I need your input to know whether my extracted RNA samples have genomic DNA contamination present or not and how the integrity of the samples is? I have nice 28s and 18s bands and the RNA...
05 May 2014 4,794 19 View
I have a problem with not properly transferring proteins on Nitrocellulose membrane. The transfer protein look fuzzy and all over the membrane as you can see in the picture. The protein marker was...
11 November 2013 3,044 17 View
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06 June 2013 7,971 19 View
I have some confusion about the amount of sample normally required to load on gel during Western blot. I will be loading 50ug of sample in total volume of 20ul (including loading buffer) ....
05 May 2013 6,307 18 View
I have a problem with Reverse Transcription. Actually RT-PCR is not giving me bands after using both Clathrin and my target sequence primer sox1. I had included gDNA and got a band for my target...
03 March 2013 6,272 18 View
I will be using RIPA lysis buffer for other cells as mouse brain tissue has a lot of fats so my worry is will RIPA buffer work best on brain tissue? Another thing is if someone has any idea which...
02 February 2013 5,644 5 View