Has anyone tried to use the same quencher for 2 reporter dyes in multiplex qPCR?

I would like to set up a taqman multiplex qPCR assay. Instead of using different dyes in my probes, I think a quencher can be used for 2 reporter dyes, e.g. FAM -TAMRA and JOE-TAMRA.

01 April 2015 1,097 3 View