Aymeric Rivollier

3 Questions 2 Answers 0 Followers

Questions related from Aymeric Rivollier

How do I generate mouse TH17 cells in vitro?

I am currently having consistency issues when generating Th17 cells in vitro. In general the viability in our cultures is pretty bad (around 30%) and the percentage of Th17 cells is never higher...

03 March 2015 615 7 View

What type of plastics (plates, tubes and pipet tips) should be used while working on endotoxin quantification in biological samples?

I know for example that polypropylene materials cannot be used due to their affinity and binding with endotoxins. Can Polystyren materials be used or is it better to exclusively use glass material?

11 November 2014 5,806 0 View

Does anyone have a protocol or advice on how to avoid hemolysis while taking blood samples from a mouse?

I perform a colorimetric assay on serum prepared from these blood samples and the red color due to hemolysis is highly interfering with my readout. I tried tail vein, cardiac puncture and still...

11 November 2014 1,212 8 View