Ayda Assadi

2 Questions 2 Answers 0 Followers

Questions related from Ayda Assadi

If I heat inactive a restriction enzyme can i again add another the same restriction enzyme to the same sample?

if after cutting i realize that the digestion (and heat inactivation if enzymes) isn't complete, can i again add the same enzyme and continue the digestion reaction?

08 May 2021 7,329 3 View

PUC or pET vectors for Geobacter sulfurreducens?

I am working with Geobacter sulfurrefucens and I have access to a limited collection of plasmids. does anyone know that pET plasmids or pUC plasmids works for overexpression in this bacteria?

26 October 2020 4,202 3 View