Austin Paul Wright

3 Questions 9 Answers 0 Followers

Questions related from Austin Paul Wright

Does anyone have the sequence for pKD78?

I am trying to design primers so that I can check my transformations. I am using lambda red mutagenesis to knock out a gene and I have to use the pKD78 vector but I can only find the sequence for...

01 January 2017 2,268 1 View

Really bad resolution and smearing in agarose gel?

For some reason my gel today came out very weird. I ran a 1% agarose gel with EtBr added before pouring. I used 1x TAE ran for 60 using 60 V. The ladder is a 50 bp ladder and the band I am looking...

02 February 2016 2,807 8 View

How to spin bacterial cells without killing them?

I am trying to do a gene expression assay and I have to wash the cells in starvation media. I am trying to use a centrifuge instead of a filter to save a lot of money. What is the best way to spin...

02 February 2016 3,432 4 View