Austin Meier

4 Questions 9 Answers 0 Followers

Questions related from Austin Meier

Does the qPCR reaction volume overall effect on CT?

I am struggling right now with my one-step qPCR for my two GOIs. They seem to amplify at a very high CT, and this leads to an impossibility in getting a decent standard curve. At full...

24 June 2014 8,855 8 View

Can you help me with Amplification in NTC (only for some primers) RT-qPCR?

Okay, I know the obvious answer is primer dimers, but I looked at the melt curves, and I am seeing the expected Tm. But I have been getting amplification in my NTC controls in my qPCR. The Cts...

20 June 2014 1,085 2 View

Diluting RNA for one-step qPCR

I was struggling with the standard curves for my RT-qPCR, and my PI suggested trying one-step instead of 2-step. After diluting one of my RNA samples to generate a standard curve (started with...

04 June 2014 1,199 1 View

Can anyone offer advice on using qPCR to compare homolog expression?

Quick background: I want to compare the expression of two homologs in potato. The two genes are >80% identical, so designing primers has to be a tricky process. I have designed a few of my own,...

14 May 2014 4,398 7 View