Augustine Thambaiah

4 Questions 6 Answers 0 Followers

Questions related from Augustine Thambaiah

FBS from which country is suitable for production of GMP Clinical grade cell therapy products?

MSC expanded using FBS as supplement is being used for various clinical trials. Which is the most acceptable FBS (country of origin) for production of clinical grade cells in a GMP facility?

05 March 2018 4,188 2 View

What is the population doubling time of mesenchymal stem cells derived from human bone marrow derived?

while calculating PD from which passage number should we take into consideration? Passage 0 (culture initiation) will not have the absolute MSC cell count since we just add Mononuclear cells from...

06 April 2015 8,787 5 View

Does media change or passaging of cells in cell culture reduce endotoxin levels?

Does culture passaging or media change reduce endotoxin levels?

30 December 2014 9,511 12 View

CFSE Cell trace proliferation assay to check immunomodulatory property of MSC cultured with different supplements?

I have MSC cultured using different supplements. To check if the change in supplement affects the immunomodulatory property of MSC, we are planning to perform CFSE T CELL PROLIFERATION ASSAY. I...

29 August 2013 4,921 0 View