August Brookwell

6 Questions 3 Answers 0 Followers

Questions related from August Brookwell

What are some statistical tests I can run on time series data?

I am measuring the expression of a fluorescent protein over a period of 4 hours (15 min intervals), testing 4 different conditions with 2 control groups (one positive for expression of the...

24 March 2023 1,959 4 View

Tips for successful PCR of amplicon over 1kb?

I am attempting to amplify out a 1375bp segment of gDNA and I'm wondering what the ideal PCR parameters would be. On my first attempt, I ran a touchdown pcr and subsequently ran the samples on an...

01 May 2021 7,194 4 View

Recommended culture volume/pellet mass for BL21(DE3) genomic DNA extraction?

I am using the NEB Monarch Genomic DNA Purification Kit to extract gDNA from BL21(DE3) cells. The protocol says to use a maximum of 2x10^9 cells but does not mention a recommended culture volume...

26 April 2021 4,157 2 View

When designing mutagenesis primers, what is the difference between those that anneal back-to-back compared to those that have overlapping 5' ends?

For site directed mutagenesis primer design, specifically those designed to make single-nucleotide substitutions, I have seen methods that utilize back-to-back primer pairs that anneal at the 5'...

12 March 2021 8,235 6 View

Can Streptomycin be Inhibited via camR?

I know that camR inhibits Chloramphenicol via expression of chloramphenicol acetyltransferase, but could that transferase also inhibit Streptomycin, or maybe interact with it in someway that...

06 February 2021 5,740 1 View

What is the danger of adding too much protease inhibitor in your lysate during purification?

During protein purification, a protease inhibitor is added to the lysate (10ul/mL of lysis buffer). Why is this amount of inhibitor desirable? What is the potential danger to the protein if a...

24 November 2020 8,351 7 View