Audrey McAlinden

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Questions related from Audrey McAlinden

What is a good normalizing gene for proliferating/differentiating cells and terminally differentiated cells?

We work with chondrocytes and finding it difficult to determine a suitable normalizing gene for proliferating and terminally differentiated (hypertrophic) chondrocytes. We want to compare gene...

06 June 2014 5,627 1 View

What's the best normalizer for miRNA RT-PCR assays? RNU44, U6 or snoRNAs?

I realize the answer to this may be more cell-type specific. We use human MSCs as well as human/mouse chondrocytes. Just wondering if anyone has had luck with a specific normalizer that works well...

06 June 2014 7,862 7 View