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Questions related from Audrey Kow
I've tried inducing active systemic anaphylaxis based on a paper. Briefly, rats were sensitised with 10 microg of OVA + 10 mg of alum on Day 1 followed by induction with 1 mg of OVA on Day 14....
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Been doing beta-hexosaminidase release assay but the results hasn't been promising. Firstly, there's no colour development in the supernatant and secondly the release has been below 10% for all...
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How to best maintain RBL cells? What is a good range of passage number to be used for assays?
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Planning to do a MTT (cytotoxicity) screening of a plant extract. Is there an acceptable range of concentration? Is 100mg/ml acceptable?
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My DMEM media and cells' supernatant turned slight yellow upon addition of freshly prepared Griess reagent (1% sulphanilamide + 0.1% NED in 2.5% phosphoric acid). Has anyone encountered such...
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