Atreyi Ghatak

3 Questions 3 Answers 0 Followers

Questions related from Atreyi Ghatak

What is the best kit/method for RNA extraction/isolation from mouse tumor samples?

We are planning to extract RNA from mice tumors. Our downstream application will involve doing quantitative PCR (from cDNA). Does anyone have experience what is the best protocol of RNA isolation...

10 June 2019 2,236 6 View

Can anyone suggest an appropriate dose of doxycycline in feed for tet-on system in vivo?

We are working with a tet-one inducible expression system, and we have been using Doxycycline for over expressing our gene of interest. We now want to move to in vivo work and would like to use...

02 April 2019 8,481 0 View

Has anyone got experience working with R&D systems human methyl cellulose media?

I am trying a clonogenic assay with PC3 (prostate cancer) cells. Since I am trying to standardize the conditions for the assay first, I am not using any treatment at this point; instead I am...

23 August 2018 2,803 1 View