Atena Sadat Sombolestani

4 Questions 3 Answers 0 Followers

Questions related from Atena Sadat Sombolestani

What is PCR bias?

I've recently faced a question that I couldn't solve on my own or with colleagues. I want to know what are PCR biases and how to handle them? Any answer in welcome.

21 June 2023 9,239 1 View

Questions about the bacterial taxonomy ?

Hi all, I have two questions regarding taxonomy 1- how do you define a novel family? or even order? 2- if you have a strain that is not deposited in two culture collection, can you describe it...

21 June 2023 6,305 1 View

Gram negative inhibitor in culture media?

I want to isolate only yeasts from a sample and I want to know if there is (a) compound/compounds that I can use to inhibit the growth of gram-negative bacteria in my culture media. If that...

07 October 2021 2,957 6 View

DNA purification post extraction for kombucha?

I extracted DNA from some kombucha products with a manual protocol, with one final step using phenol, for 16S and ITS sequencing. However, there is a problem for the majority of my samples in PCR...

13 June 2021 9,602 3 View