Asim Haque

5 Questions 4 Answers 0 Followers

Questions related from Asim Haque

Why HEK 293TT don't grow in Roller bottle?

I was culturing HEK 293 TT (from ATCC) in 75-TCF. After two passages in flasks finally I passage the cell in Roller bottle. But next day I found not cell attached and clot together. I have no...

15 July 2025 3,298 1 View

How could I break DNA using water bath sonicator?

I have tried to break DNA by water sonicator, but after gel run i saw that nothing happen. I took 300 μl DNA in eppendrop and flaot it on the bath, temp was 28-34`C, i sonicate the sample in three...

16 August 2021 4,715 4 View

How can charactarize the contamination?

I was culturing vero cell in Roller Bottle. After 2 days of incubation i noticed white / creamy patches attached to inside surface of the bottle. Upon shaking the patch detach from the surface...

05 September 2020 4,417 7 View

Contamination Burden in an Animal cell culture lab in case of a Microbiology lab beside, beyond handling error. Suggestion and opinion required.

# Animal cell culture without using any antibiotic requires more care fullness. But if recurrent contamination is happening and there is no deal with the sterility of media, reagents and...

01 January 1970 4,559 3 View

HEK 293TT don't grow in Roller bottle

I was culturing HEK 293 TT (from ATCC) in 75-TCF. After two passages in flasks finally I passage the cell in Roller bottle. But next day I found not cell attached and clot together. I have no...

01 January 1970 8,349 1 View