2 Questions 3 Answers 0 Followers
Questions related from Asim K Bepari
I am trying to detect protein phosphorylation by IP-IB. To prepare tissue lysates I am using RIPA lysis buffer which contains EDTA . I add Sodium Orthovanadate as a phosphatase inhibitor. I see in...
03 March 2017 6,462 3 View
I have been using COS1 and BHK-21 cell cultures. I coat the coverglasses with Poly-DL-ornithine (Sigma). Cell attachment is poor for BHK-21 and fair for COS1. Now I am moving to Poly-l-lysine +...
23 October 2015 2,840 4 View
