Asif Raza

4 Questions 6 Answers 0 Followers

Questions related from Asif Raza

Alternative for Pulse-Chase experiment?

Dear All, I need to study the biosynthesis of a particular protein after the removal of the inhibitor. Is there any non-radioactive alternative is available to track the biosynthesis of newly...

09 November 2020 1,330 2 View

What are the various sets of experiments one can perform to prove a particular gene is silenced by Histone deacetylation/chromatin remodeling?

After treatment with a particular drug, my target gene starts expressing. What are the various ways or experiments I should perform to confirm that the particular gene was suppressed by Histone...

12 March 2015 8,511 3 View

Cell lysis buffer composition for GST tagged protein purification in soluble fraction?

The protein I am working on is quite large (Approx 70kDa, pI-8.96). It is an animal cell membrane protein I am expressing in E.coli. I used the cell lysis buffer (PBS (pH-7.4), PMSF, EDTA and...

30 October 2013 3,171 11 View

Problems with Transformation / DH5-alpha / Plasmid DNA

We have cloned a gene in vector pVITRO2 (GFP-HYG-LacZ; 10KB), and transformed it into DH5-alpha E.coli cells with suitable antibiotic (of course in the right concentration). The problem is, after...

24 May 2013 9,819 14 View