Ashwini Challa

2 Questions 2 Answers 0 Followers

Questions related from Ashwini Challa

What is the Optimum duration for DOX treatment to induce shRNA mediated Knockdown in HCT-116 cells using pLKo-Tet-On system?

Hello all: I am trying to generate Knock downs in HCT-116 using pLKO-Tet-On system by treating the HCT cells with DOX (1ug/ml) for three days to induce shrNA. I can only see a minute decrease of...

13 February 2018 6,139 1 View

Any suggestions on Transducing HCT-116 cells using lentivirus with pTet-On vector??

Hello all: This is my first time working with lentivirus and transductions. I am trying to generate KD HCT-116 cell lines using shRNA in pTET-On vector. Can anyone answer me the following...

06 December 2017 2,990 3 View