Ashwin Wadaskar

4 Questions 4 Answers 0 Followers

Questions related from Ashwin Wadaskar

Can anybody please suggest electropoaration buffer for leukemic cell lines?

We want to transfect leukemic cells KG1a and K562 with a plasmid DNA by electroporation. We are looking for commercially available electroporation buffer, can anybody recommend some good...

03 March 2017 7,946 3 View

Why is acetone wash important during TCA (trichloroacetate) precipitation?

Why is acetone wash important during TCA (trichloroacetate) precipitation of proteins durinf ELISA and western blotting?

01 January 2016 7,220 0 View

Why do we use 1% BSA for primary antibodies in western blotting?

why do we use 1% BSA for primary antibodies in western blotting?

11 November 2015 8,350 7 View

I want to find miRNA target genes in leukemia. Can somebody tell me how to use online miRNA databases for such study?

How to identify miRNA target genes using online databases?

01 January 1970 3,468 2 View