Ashok Kumar Mallik

5 Questions 11 Answers 0 Followers

Questions related from Ashok Kumar Mallik

How to delimit species boundary based on genetic distance ?

If A and B are two morphospecies (sisters) separated by lower genetic distance ( of 3% in cytb locus) then can we use this lower value as a “cut off value ” for other sets of sister lineages (say...

26 September 2016 3,759 7 View

How to treat gaps in 16S ribosomal RNA in multiple sequence alignment?

I am working on a few snake genera. In my 16S multiple sequence alignment I get gaps with ambiguously aligned region in alignment matrices, which happens to be part of 16S loop structure. It seems...

13 January 2014 543 5 View

How to get rid of pseudogene amplification?

I am working for a snake genus and I amplified multiple samples of the same species with a set of ND4 primers used in other 90% of the studies on snakes. However, when I cross checked for...

09 January 2014 916 3 View

What is the best strategy to sample a widely distributed species for phylogeny?

Currently, I am working on few widely distributed taxa in Indian landmass and trying to build phylogeny. I would like to know, whether my phylogenetic result will be biased if I collect samples...

01 January 2014 9,645 6 View

How to generate consensus DNA sequence (contig) from forward and reverse sequence? Which software will I use?

After generating the sequence, how can I confirm that the desired contig sequence is perfect? Currently I am trying to use "bioedit" to generate my desired sequence from both reverse and forward...

04 November 2012 6,659 83 View