Ashley Parker

4 Questions 5 Answers 0 Followers

Questions related from Ashley Parker

What are these shadows appearing in my Western Blot?

I'm relatively new to Western Blot and I recently blotted for pCREB vs CREB in my samples. When I showed the blot to my PI, she asked what these background bands were in my blot, as she'd never...

21 October 2021 9,645 5 View

Best Coating for Assessing Neuronal Morphology in vitro?

I am studying neurodevelopment using primary cerebellar granule neurons (CGNs) with a focus on studying morphology. CGNs undergo dynamic morphological changes in vivo and in organotypic slice...

01 August 2019 8,471 1 View

How to identify cerebellar granule cells using hemocytometer?

I am preparing enriched CGN cultures, meaning I have a mixed population of neuronal cells but with the majority being Granule cells. Our automatic cell counter was not getting accurate numbers due...

16 July 2019 2,813 0 View

How to get a physiological pH when making 16% Paraformaldehyde?

I made 16% paraformaldehyde from scratch (dissolving powder in ddH20). While I was attempting to pH it, it would rapidly switch from alkaline to acidic according to the pH strips we use (range pH...

09 April 2019 6,405 3 View