3 Questions 5 Answers 0 Followers
Questions related from Aseel Marahleh
First Q: Most protocols allow for 1-5% FBS in FACS buffer solution, however, I use 10% FBS and I never had problems with the sort. what is the reason behind the reduced FBS? Second Q: Is it okay...
23 April 2020 9,383 5 View
In measuring ERK1/2 levels in a homogenous sample, what can contribute to increased ERK1/2 levels apart from loading different amounts of total protein or the phosphorylated state of ERK1/2. So...
12 October 2019 2,120 5 View
My question is about the storage of proteins (to probe for phosphorpoteins) after adding SDS buffer and B-meracptoethanol, reducing for 5 minutes at 95c, is the sample going to be stable at -20C?...
02 August 2019 3,242 4 View
