Arwa Al

3 Questions 12 Answers 0 Followers

Questions related from Arwa Al

What is the differences between Methylation-Specific PCR and Sequencing after bisulfite treatment primers and how to design both?

I have a primer for a Methylation-Specific PCR (MSP) taken from the previous study and I want to design a primer for sequencing bisulfite-converted DNA for the same region that MSP primer bind to...

05 May 2017 8,839 7 View

What should be the differences and the range between tm of primers and annealing in RT-PCR?

If Tm of the both primer was 67C, is it 60C perfect as annealing temperature?

04 April 2017 630 12 View

Possibilities that made my RT-PCR wrong?

Hello, I would ask about RT-PCR for 4 different genes including GAPDH for control sample and the ct value gave above 37 cycle up to 40 in different concentrations of primers so what is the...

11 November 2016 6,106 11 View