Arusha Acharyya

4 Questions 5 Answers 0 Followers

Questions related from Arusha Acharyya

Is the protein concentration (when the unknown sample is not BSA) determined from Bradford Assay accurate?

I am using Bradford Assay to determine the unknown concentration of a protein. I have used BSA standard solutions to get a standard curve and then use it to determine the concentration of my...

27 August 2021 915 7 View

How to determine the error limits in DLS measurements?

I usually do 5 measurements with 10 acquisitions each time to get size distributions for a sample. However, I am curious about the intrinsic resolution of the instrument (I use a Wyatt DyanaPro...

12 August 2021 6,748 3 View

What is best way of preparing protein fibril sample for AFM analysis on mica surface?

I am having difficulties to image protein fibrils using AFM. What is the ideal concentration of amyloid fibrils that I should use? Also how long does it need to sit on the mica surface before I...

14 January 2019 5,794 6 View

What is the best way to remove air cavities from an aqueous solution when doing transient absorption measurements?

I am doing transient infrared absorption experiments with aqueous solution of a protein. My problem is that I am not getting proper signal due to cavity formation from the dissolved air in the...

21 October 2016 9,829 5 View