Aruna Raja

2 Questions 5 Answers 0 Followers

Questions related from Aruna Raja

Oxidative assay (DCF) in differentiated SH-SY5Y cells protocol?

I need to detect the ROS levels of the differentiated neuronal cells using DCFDA reagent from Thermofisher. Following are the steps followed: 1. Differentiated neuronal cells seeded in 96-well...

03 December 2018 1,700 3 View

Pull Down Assay to check DNA-Protein interaction?

I am trying to carry out pull down assay using magnetic beads with single stranded biotinylated DNA of interest. Image attached. Lane 1: marker Lane 2: Beads only Lane 3: Protein + Biotin DNA +...

02 November 2018 7,782 3 View