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According to Kramer publication (Curr Protoc Mol Biol. PMC 2012 July 1) It is mentioned under "SL RT primer" Transfer to new tube, then overlay with 100 μL molecular-biology grade mineral oil....
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I made the siRNA and Lipofectamine2000 mix and incubated 20 minutes @ room temperature. The problem now is the cells are not healthy to start the transfer. Anybody please let me know how long i...
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