Arun Kumar

4 Questions 12 Answers 0 Followers

Questions related from Arun Kumar

Can someone help me in troubleshooting EMSA problem?

I am using LightShift Chemiluminescent EMSA (Thermo Scientific, USA) kit for showing DNA-protein interaction of a transcription factor to promoter of a gene. A double stranded 30 bp...

19 August 2015 5,452 7 View

Is prospecting thermostable enzymes from plants a better option over thermophilic microorganisms?

I am working on thermostable superoxide dismutase (SOD) and isolated SOD from few Plant sp. which are resistant to heat denaturation at high temperatures of 80 °C and above. There are reports on...

25 October 2012 3,714 3 View

How can the thermostability of enzymes from plants be correlated to evolutionary adaptations?

I am working on thermostability aspects of plant superoxide dismutases (SODs). Thermostability varies between plant species under study. I wonder how thermostable SODs evolved in plants. Is...

12 October 2012 440 5 View

Mutating Cysteine to Alanine instead of Serine. Why?

What can be the possible advantage of mutating Cysteine to Alanine instead of Serine which is the closest replacement for Cysteine? I see many articles where Cysteine has been replaced with...

06 March 2012 4,614 11 View