Arun Balasubramaniam

3 Questions 4 Answers 0 Followers

Questions related from Arun Balasubramaniam

Which is the best method to knockout/isogenic mutation in Staphylococcus bacteria?

I want to knockout a specific gene present in the S. epidermidis ATCC 12228 strain. Kindly suggest me the efficient methods that weren't lethal to bacterial cells. Thanks in advance. Arun AB

28 August 2020 5,897 3 View

Why it needs long time exposure to develop/get to see band images in western blot?

Hello Everyone, I'm checking expression for 4-HNE (abcam) (1:2000) by western blotting in mice skin tissue against UV stress. I'm facing struggle to get good visible bands, as I get band view only...

19 March 2020 5,938 3 View

Can we use DNA as template to do real-time PCR to check expression of gene?

Hello Everyone, I wanted to run RT-PCR using onestep real-time PCR systems and SYBR green (ThermoFischer) to check the gene expression in bacteria. I wanted to directly use 50 ng of DNA samples...

11 March 2020 2,088 3 View