Arman Akşit

6 Questions 17 Answers 0 Followers

Questions related from Arman Akşit

Could energetically unfavourable dyad symmetry be the reason for the low expression level of my protein of interest?

Dear all, I have cloned a plasmid for the expression of an artificial protein which has many repetitive sequences (mimicking native protein of interest). However, the protein is not produced (or...

01 January 2019 2,181 10 View

How can I convert a replicative plasmid into integrative plasmid?

Hi all, I would like to clone one gene with a fluorescent tag into a vector containing NatMX marker. In my current lab, we only have pFA6-NatNT2 plasmid....

10 October 2018 1,537 5 View

What are the examples of non-metabolic pathways?

In biochemistry, a metabolic pathway is a linked series of chemical reactions occurring within a cell. The reactants, products, and intermediates of an enzymatic reaction are known as metabolites,...

05 May 2018 1,299 5 View

How to read a phylogenetic tree?

I have a phlyogenetic tree (see attachment). In the legend, it is written that the distance scale represents the number of differences between the sequences. My question is how can I...

05 May 2018 9,558 10 View

What is the difference between cortical and peripheral ER?

I am getting confused with these terms. Are they represent the same part of the ER? It is written "The cortical endoplasmic reticulum (ER) is an intricate network of tubules and cisternae...

05 May 2018 8,781 3 View

How can I find if a peptide sequence contains any targeting signals or motif?

I constructed a plasmid which allows expression of a fusion protein (Protein A+Tag+ProteinB). Sequencing of the plasmid showed that there is a single nucletiode insertion in "Tag" which causes...

09 September 2016 6,434 7 View