Arjen Boender

3 Questions 12 Answers 0 Followers

Questions related from Arjen Boender

Anybody here that routinely performs northern blots?

Since I get an extreme amount of products when doing PCR and 3'-UTR RACE for my gene of interest I am thinking of turning to the old-fashioned northern blot to increase sensitivity. I need to...

08 May 2016 5,266 6 View

How can you get PCR amplification of only half the sequence that you expect (not considering non-specific amplification)?

So the situation is as follows: I am studying a gene of which a particular isoform has been described that lacks exon 9. I designed primers around this exon so that after PCR reaction I should get...

23 March 2016 9,359 3 View

Any experts on post-transcriptional modulation of mRNAs?

I am interested in the expressional regulation of an mRNA that has three different isoforms: one with almost no 3'-UTR and two with 3'-UTRs of about 6000 nucleotides. I am wondering what I can...

24 June 2015 3,775 6 View