Arjen Boender

4 Questions 13 Answers 0 Followers

Questions related from Arjen Boender

Anybody has experience with AAV-production kits? Are they working well?

I want to speed up my AAV-production process and saw that they are some kits around that promise me that very goal (from Clontech, SBI, etc.). Anybody has experience with these kits? Pros and...

12 March 2018 4,442 2 View

Anybody here that routinely performs northern blots?

Since I get an extreme amount of products when doing PCR and 3'-UTR RACE for my gene of interest I am thinking of turning to the old-fashioned northern blot to increase sensitivity. I need to...

08 May 2016 5,307 6 View

How can you get PCR amplification of only half the sequence that you expect (not considering non-specific amplification)?

So the situation is as follows: I am studying a gene of which a particular isoform has been described that lacks exon 9. I designed primers around this exon so that after PCR reaction I should get...

23 March 2016 9,408 3 View

Any experts on post-transcriptional modulation of mRNAs?

I am interested in the expressional regulation of an mRNA that has three different isoforms: one with almost no 3'-UTR and two with 3'-UTRs of about 6000 nucleotides. I am wondering what I can...

24 June 2015 3,820 6 View