6 Questions 14 Answers 0 Followers
Questions related from Ariel Waisman
Hi! I am trying to inhibit Chk1 kinase phosporilation upon DNA damage. I was thinking on using the inhibitor AZD-7762. What is the best way to evaluate that the AZD is working? Should a western...
01 January 2016 2,098 3 View
I am doing this cell sorting experiment, which lasts around 1 hour and a half. The cells are resuspended in FACS buffer (PBS -/-, 0,1% BSA, 20 mM HEPES) and kept on ice until sorting. After...
06 June 2015 4,389 14 View
Hi! I am trying to reversibly inhibit DNA replication of my mouse cells. I read that a lot of inhibitors actually cause DNA crosslinking and eventually lead to apoptosis, which I don't want. I am...
06 June 2015 5,096 9 View
I've been trying to set up a NSC differentiation protocol for a while using the 46C mESC, which contains a GFP/Puro reporter under the control of the Sox1 NSC marker gene, as well as with other...
11 November 2014 6,470 7 View
I want to detect mKO2 stably expressing cells with the flow cytometer, but we only have the 488 nm and the 633 nm lasers, and not the 561 nm laser. According to the spectrum, the fluorophore is a...
07 July 2014 2,898 3 View
I am trying to make a knock-in using CrispR/Cas system. For that, I would need a targeting vector in which I could insert the two homology arms flanking the sequence I want to add. Is it necessary...
04 April 2014 3,630 16 View
