Ariel Waisman

4 Questions 10 Answers 0 Followers

Questions related from Ariel Waisman

Why don't mESCs attach when growing in defined conditions using N2B27+Lif+2i?

I am trying to grow mESCs in defined contitions using N2B27 + 2i + LIF. I was growing the cells in these conditions in another lab and it worked perfectly well, but when I try it in my lab cells...

16 May 2016 2,108 9 View

What is the best way to evaluate that the AZD-7762 inhibitor is working on Chk1 upon DNA damage?

Hi! I am trying to inhibit Chk1 kinase phosporilation upon DNA damage. I was thinking on using the inhibitor AZD-7762. What is the best way to evaluate that the AZD is working? Should a western...

06 January 2016 1,580 3 View

Does anyone have experience transporting IPSC cells by plane?

I am traveling to the US and I have to take a few mouse IPSC lines with me. The problem is that we can't send them in dry ice, so I've been told that many people take them directly in the luggage....

27 October 2014 7,267 6 View

Is there a database with easily available and already processed RNA-seq data for Pluripotent Stem cells and their derivatives?

I am looking for a tool to easily analyze the expression of different genes during the differentiation of mouse or human pluripotent stem cells to different derivatives, something similar to...

01 January 1970 9,802 3 View