I have performed the cell-free Griess assay, using Sodium nitroprusside to obtain the NO, but the absorbances I obtain are too low!

I want to perform de Griess assay without cells, therefore I have used Sodium Nitroprusside (SNP) which in PBS forms espontanously NO.  I have added 50 uL in a 96-well plate of SNP 10mM and added...

03 August 2017 5,973 2 View