Arezoo Setayesh

6 Questions 8 Answers 0 Followers

Questions related from Arezoo Setayesh

How to get consistent result from CD spectroscopy?

I am doing CD studies on amino acids by measuring the CD intensity (quantative) for various concentrations of amino acids both when they are pure and when they have been used as precursors for...

08 December 2021 9,210 7 View

How to differentiate the emission peak of a compound from the emission peak of the light source(instrument)?

I am having trouble recognizing whether the emission picks that I am seeing are from the light source of the instrument or from my sample?? is there a way to better discriminate them? (I am not...

22 November 2021 9,345 3 View

Is there another way to eliminate EDC/NHS out of the system except precipitation?

I tried precipitation with cold acetone several times but it didn't work. I tried to use dialysis for several days but my H-NMR analysis shows traces of NHS. How can I get rid of the reagents...

22 January 2018 1,074 8 View

Is it normal to form a foam structure in EDC/NHS reaction?

when I add the molecule carrying the amine functional group to the solution consisting the EDC/NHS and the molecule carrying the carboxyl group, it forms a foam structure and it's kind of strange...

29 December 2017 153 1 View

Is it possible to use Hydrochloric acid to regulate the pH for MES buffer?

I have the salt for making the MES buffer ( 2-(N-morpholino)ethanesulfonic acid ) and 0.1M solution of it give me a PH of almost 7 which I have found out it's due to the DI water( I used to make...

26 December 2017 3,426 2 View

Mol ratio of EDC/NHS is based on the mol of the carboxyl group or amine group?

I'd like to use EDC/NHS in an excess platform to conjugate an amine group to the carboxyl group of a polysaccharide. Considering this fact that i don't have the optimize mol ratio for my...

19 December 2017 5,706 7 View