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What Happen if we skip the end repair step in exome library preparation or the enzyme doesn't work properly for that step?
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I am working with SureSelect XT2 all exons-library prep protocol and I am getting different yields every time I am using this protocol. I am a little uncertain about the first Ampure clean up step...
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The results of quality control for one of my amplified libraries showed two peaks. The one at 200 was what I expected but I don't know what is the second one and if that might cause problem with...
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