Archishman Ghosh

5 Questions 8 Answers 0 Followers

Questions related from Archishman Ghosh

How to fit data to the partial differential equation for 1-D diffusion using MATLAB?

I have some data represented as a surface below with time and distance axes. I would like to fit this to all possible solutions for the diffusion equation that is: dS/dt = D.d2S/dx2 where S is...

13 May 2021 3,313 2 View

Does fluorescence intensity of a dye depend on viscosity? How does one correct for this?

In highly viscous media the rotational motion is impeded. Does this cause fluorescence intensity to rise? What's the mechanism for this? Also, how would I correct for this artificial viscosity...

07 February 2019 6,481 2 View

Can a 12 kD protein flow through a 3500 MWCO spin concentrator if spun long enough?

I have an intrinsically disordered protein which I spun for several hours in a 3500 molecular weight cut-off spin concentrator at about 2000xg. Can the protein slip through the pores being a...

21 September 2017 1,539 2 View

What can I use to keep my protein from aggregating?

I have a disordered protein that is tagged with MBP with a TEV cleavage site. The protein is has a formal charge of +22 at pH 7. The moment I cleave it from MBP it aggregates with what I assume is...

03 November 2016 3,333 15 View

How do I estimate the Kd of a high affinity protein using only instrinsic tryptophan fluorescence spectroscopy?

My protein has a Kd of around 4-5 nM. It is very difficult to bring down the concentration of the protein below that. It is not showing proper results at higher concentrations. I was hoping to use...

18 May 2014 8,500 15 View