Aravinth S.

2 Questions 2 Answers 0 Followers

Questions related from Aravinth S.

NIH 3T3 transfection protocol?

Hi, Can anyone share their transfection protocol for NIH 3T3 cells. I tried with lipo3000 protocol. Very few cells are transfected and their shapes change after transfection.

30 November 2021 6,582 1 View

What will happen if we keep the at low temperature (100-200 K) cell after fixation?

For Fluorescence imaging after the transfection the cells are fixed and the protocol says that the fixed cells(within the coverslip) needs to be stored at 4o C. What will happen if we keep the...

11 October 2021 996 2 View