Arad Bustan

2 Questions 3 Answers 0 Followers

Questions related from Arad Bustan

Is it possible that Melting peak is below 80°C when amplicon is above 500bp?

I have seen this phenomenon. Qpcr product band size was validated by Gel electrophoresis. Melting peak tempreture of my product is 79°C.

01 October 2020 492 3 View

How can I compare of genes that are expressed extremely low. in these cases, which technique do you recommend that remove primer dimer melting peaks?

Recently, I have been investigating of expression of Sall-4 in two different types cancers. Due to of acceptable melting peak of Beta Actin(and Cq=17), I am sure the quality of cDNA is pretty...

09 August 2020 3,134 8 View