April Pawluk

4 Questions 23 Answers 0 Followers

Questions related from April Pawluk

Can anyone tell me how to construct a taxonomic tree of all genera of proteobacteria?

I don't need to go to the species level, just the genus level, and I would like to make a hierarchal taxonomic tree to show from phylum (proteobacteria) down to each genus. My plan is to then...

07 July 2015 9,043 10 View

Has anyone used E. coli cell free extracts for production / purification of "difficult" proteins?

I am looking for a way to produce high 100ug to 1mg amounts of proteins that are insoluble expressed in E. coli. I thought that cell free extracts might be a way to do this. These are bacterial...

06 June 2014 4,967 0 View

Does anyone have experience with co-transformable vector systems for Pseudomonas aeruginosa?

In our lab, we have a set of vectors we can use in P. aeruginosa, however all the vectors have the same origin and so we can't co-transform two plasmids into the same cell. I'm wondering if anyone...

04 April 2014 5,185 3 View

Does anyone have experience with "SoluBL21" cells from Genlantis?

I have a few proteins that I have not been able to purify from BL21(DE3), other E. coli host strains, or their original host P. aeruginosa, due to insolubility. I get great induction of the...

04 April 2014 1,153 15 View