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Questions related from April Pawluk
I don't need to go to the species level, just the genus level, and I would like to make a hierarchal taxonomic tree to show from phylum (proteobacteria) down to each genus. My plan is to then...
07 July 2015 9,048 10 View
I am looking for a way to produce high 100ug to 1mg amounts of proteins that are insoluble expressed in E. coli. I thought that cell free extracts might be a way to do this. These are bacterial...
06 June 2014 4,971 0 View
In our lab, we have a set of vectors we can use in P. aeruginosa, however all the vectors have the same origin and so we can't co-transform two plasmids into the same cell. I'm wondering if anyone...
04 April 2014 5,190 3 View
I have a few proteins that I have not been able to purify from BL21(DE3), other E. coli host strains, or their original host P. aeruginosa, due to insolubility. I get great induction of the...
04 April 2014 1,158 15 View