Anwesha Kar

2 Questions 2 Answers 0 Followers

Questions related from Anwesha Kar

What is the optimum time and concentration of Micrococcal nuclease digestion and the sonication pulses required for Digestion of DNA for ChIP?

Hi Everyone, I am trying to optimize ChIP for sorted CD8 T cell obtained from mouse splenocytes(primary cells). I am doing the ChIP with a kit from Cell Signaling Technologies[SimpleChIP®...

22 December 2020 8,538 3 View

Is it possible to stain T cell with Bodipy and FillipinIII simultaneously? If so, can anyone give me the protocol for the staining in Flow Cytometry?

I am trying to quantify the Plasma Membrane cholesterol, total cellular cholesterol as well as the the amount of Cholesterol esters inside the cell by Amplex Red Cholesterol Assay Kit. I have also...

19 July 2020 5,832 0 View